Hauv kev tshawb fawb niaj hnub no, protein electrophoresis yog ib txoj kev siv tseem ceeb rau kev tshuaj xyuas thiab piav qhia txog cov protein. Lub plawv ntawm txoj kev no yogPolyacrylamide, ib qho tshuaj sib xyaw ua ke uas ua haujlwm ua tus txha nraub qaum ntawm cov gel matrices siv hauv cov txheej txheem gel electrophoresis. Cov khoom tshwj xeeb ntawm Polyacrylamide ua rau nws yog ib qho cuab yeej tseem ceeb rau cov kws tshawb fawb thiab cov kws tshawb fawb uas tab tom nrhiav kev daws qhov nyuaj ntawm cov protein thiab lawv cov kev sib cuam tshuam.
Polyacrylamide, feem ntau hu ua PAM, yog ib qho polymer tsim los ntawm acrylamide monomers. Nws qhov kev hloov pauv zoo kawg nkaus yog vim nws muaj peev xwm tsim cov saw ntev, ua rau muaj cov khoom zoo li gel uas tuaj yeem haum rau ntau qhov ntau thiab tsawg ntawm cov molecules. Cov yam ntxwv no ua rau polyacrylamide yog tus neeg sib tw zoo tshaj plaws rau kev tsim cov porous matrices siv hauv protein electrophoresis.
Protein electrophoresis yog ib txoj kev uas cais cov protein raws li lawv lub zog thiab qhov loj. Los ntawm kev muab cov qauv protein rau hauv lub zog hluav taws xob hauv polyacrylamide gel matrix, cov protein txav mus los ntawm cov gel ntawm ntau qhov sib txawv, ua rau muaj cov kab sib txawv uas tuaj yeem soj ntsuam thiab ntsuas tau. Qhov kev sib cais no muab cov kev nkag siab tseem ceeb rau kev ntshiab ntawm cov protein, kev txiav txim siab qhov hnyav molecular, thiab qhov muaj cov isoforms.
Lub Luag Haujlwm ntawm Polyacrylamide hauv Protein Electrophoresis
Qhov kev xaiv ntawm polyacrylamide rau cov protein electrophoresis yog los ntawm nws qhov xwm txheej uas hloov tau. Cov kws tshawb fawb tuaj yeem hloov kho qhov concentration ntawm cov gel matrix kom haum rau cov protein ntawm ntau qhov ntau thiab tsawg. Cov concentration siab dua tsim cov matrices nruj dua uas tsim nyog rau kev daws cov protein me dua, thaum cov concentration qis dua siv rau cov protein loj dua. Qhov kev hloov pauv no ua kom ntseeg tau tias cov kws tshawb fawb tuaj yeem hloov kho lawv cov kev sim kom ua tiav kev sib cais thiab kev tshuaj xyuas zoo tshaj plaws.
Polyacrylamide ua ib qhoCov khoom ua kom cov dej khov
Cov txiaj ntsig ntawm Polyacrylamide nthuav dav tshaj nws lub luag haujlwm hauv gel electrophoresis. Nws kuj pom cov ntawv thov ua flocculant hauv ntau yam lag luam, xws li kev kho dej thiab kev tswj cov dej khib nyiab. Ua ib qho flocculant, polyacrylamide pab hauv kev sib sau ua ke cov khoom me me hauv cov kua, pab txhawb lawv tshem tawm. Qhov cwj pwm no qhia txog cov peev xwm sib txawv ntawm cov tshuaj thiab kev cuam tshuam dav dav rau kev tshawb fawb thiab kev lag luam.
Kev Nce Qib Hauv Polyacrylamide-Based Electrophoresis
Xyoo tas los no tau pom kev nce qib tas mus li hauv cov txheej txheem polyacrylamide-based electrophoresis. Native PAGE, SDS-PAGE, thiab ob-dimensional gel electrophoresis tsuas yog ob peb qhov piv txwv ntawm polyacrylamide qhov kev hloov pauv tau ua rau muaj kev tsim cov txheej txheem tshwj xeeb rau kev tshuaj xyuas cov qauv protein, kev hloov pauv tom qab txhais lus, thiab kev sib cuam tshuam. Cov txheej txheem no muaj txiaj ntsig zoo hauv kev tshawb fawb proteomics thiab kev siv zog nrhiav tshuaj.
Hauv kev tshuaj xyuas cov protein, polyacrylamide tshwm sim ua tus khub ruaj khov, ua rau cov kws tshawb fawb nkag mus rau hauv lub ntiaj teb nyuaj ntawm cov protein. Nws lub luag haujlwm ua lub hauv paus ntawm gel matrices hauv electrophoresis systems tsis tuaj yeem hais ntau dhau. Los ntawm kev daws cov txheej txheem kab mob mus rau kev tsim cov tshuaj kho mob tshiab, polyacrylamide-based electrophoresis txuas ntxiv mus rau kev txhim kho kev tshawb fawb. Raws li thev naus laus zis nce qib, qhov txuj ci tseem ceeb no yuav hloov pauv, ntxiv rau peb txoj kev nkag siab txog cov protein thiab lawv cov haujlwm ntau yam.
Lub sijhawm tshaj tawm: Lub Yim Hli-21-2023