Nyob rau hauv lub realm ntawm niaj hnub science, protein electrophoresis stands raws li ib tug pob zeb txheej txheem rau kev soj ntsuam thiab characterizing proteins.Nyob rau hauv lub plawv ntawm no methodology lus dagPolyacrylamide, ib tug ntau yam compound uas ua hauj lwm raws li lub caj qaum ntawm gel matrices siv nyob rau hauv gel electrophoresis systems.Polyacrylamide cov khoom tshwj xeeb ua rau nws yog ib qho cuab yeej tseem ceeb rau cov kws tshawb fawb thiab cov kws tshawb fawb nrhiav kom daws qhov nyuaj ntawm cov proteins thiab lawv cov kev cuam tshuam.
Polyacrylamide, feem ntau hu ua PAM, yog cov khoom siv hluavtaws ua los ntawm acrylamide monomers.Nws qhov kev ua tau zoo tshaj plaws yog vim nws lub peev xwm los tsim cov saw ntev, ua rau muaj cov khoom zoo li gel uas tuaj yeem ua raws ntau qhov ntau thiab tsawg ntawm cov molecules.Cov cuab yeej no ua rau polyacrylamide yog ib tus neeg sib tw zoo tshaj plaws rau kev tsim cov porous matrices siv hauv cov protein electrophoresis.
Protein electrophoresis yog cov txheej txheem uas cais cov proteins raws li lawv cov nqi thiab qhov loj me.Los ntawm kev xa cov qauv protein mus rau qhov chaw hluav taws xob hauv polyacrylamide gel matrix, cov proteins tsiv mus los ntawm cov gel ntawm cov nqi sib txawv, ua rau muaj cov kab sib txawv uas tuaj yeem txheeb xyuas thiab ntsuas.Qhov kev sib cais no muab kev nkag siab zoo rau cov protein purity, kev txiav txim siab qhov hnyav molecular, thiab muaj cov isoforms.
Lub luag haujlwm ntawm Polyacrylamide hauv Protein Electrophoresis
Kev xaiv ntawm polyacrylamide rau cov protein electrophoresis yog hauv paus hauv nws qhov xwm txheej.Cov kws tshawb fawb tuaj yeem kho qhov concentration ntawm gel matrix kom haum rau cov protein ntau qhov sib txawv.Cov concentrations siab dua tsim cov matrices nruj dua tsim nyog rau kev daws cov protein me, thaum cov concentrations qis dua yog siv rau cov protein ntau.Qhov kev hloov pauv no ua kom ntseeg tau tias cov kws tshawb fawb tuaj yeem hloov kho lawv cov kev sim kom ua tiav kev sib cais thiab kev tshuaj xyuas zoo.
Polyacrylamide as aFlocculant
Polyacrylamide's utility extends tshaj nws lub luag hauj lwm nyob rau hauv gel electrophoresis.Nws kuj pom cov ntawv thov ua flocculant hauv ntau qhov kev lag luam, xws li kev kho dej thiab kev tswj dej khib nyiab.Raws li ib tug flocculant, polyacrylamide pab nyob rau hauv aggregating suspended hais nyob rau hauv cov kua, pab kom lawv tshem tawm.Cov yam ntxwv no qhia txog qhov sib xyaw ua ke muaj peev xwm sib txawv thiab muaj kev cuam tshuam dav rau kev tshawb fawb thiab kev lag luam.
Kev nce qib hauv Polyacrylamide-Based Electrophoresis
Xyoo tsis ntev los no tau pom kev nce qib txuas ntxiv hauv polyacrylamide-based electrophoresis cov txheej txheem.Native PAGE, SDS-PAGE, thiab ob-dimensional gel electrophoresis tsuas yog qee qhov piv txwv ntawm kev hloov pauv ntawm polyacrylamide tau ua rau muaj kev txhim kho tshwj xeeb rau kev txheeb xyuas cov qauv protein, kev hloov pauv tom qab, thiab kev sib cuam tshuam.Cov tswv yim no yog qhov muaj txiaj ntsig zoo hauv kev tshawb fawb proteomics thiab kev tshawb nrhiav tshuaj.
Nyob rau hauv lub realm ntawm kev tsom xam cov protein, polyacrylamide tshwm sim raws li ib tug stalwart khub, ua rau cov kws tshawb fawb delve rau hauv lub ntiaj teb no intricate proteins.Nws lub luag hauj lwm raws li lub hauv paus ntawm gel matrices nyob rau hauv electrophoresis systems tsis tau overstated.Los ntawm unraveling kab mob mechanisms mus rau tsim kho tshiab, polyacrylamide-raws li electrophoresis txuas ntxiv mus rau kev loj hlob ntawm science.Raws li kev siv thev naus laus zis, qhov zoo nkauj zoo nkauj no yuav hloov zuj zus, ntxiv dag zog rau peb txoj kev nkag siab ntawm cov protein thiab lawv cov haujlwm ntau heev.
Post lub sij hawm: Aug-21-2023